Lablog4-41:Production of antibody fragments in Escherichia coli.

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Tomohisa Katsuda, Hiroyuki Sonoda, Yoichi Kumada, Hideki Yamaji

First author

Tomohisa Katsuda

Corresponding author

Tomohisa Katsuda

Publication Style

Journal name Methods in molecular biology


Volume, issue, pages

907 305-24


Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine–Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.