Lablog4-23:Recent Advances in Controlled Immobilization of Proteins onto theSurface of the Solid Substrate and Its Possible Application to Proteomics

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Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura, Hiroyuki Imanaka

First author

Kazuhiro Nakanishi

Corresponding author

Kazuhiro Nakanishi

Publication Style

Journal name Current proteomics


Volume, issue, pages

5 161-175


Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.