Lablog4-28：Refolding of single-chain Fv by use of an antigen-coupled column

  • 投稿日
  • 著者 bioaffinity
  • 対象DB Lablog4
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Authors

Kazutaka Ikeda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji, Shigeo Katoh

First author

Kazutaka Ikeda

Corresponding author

Shigeo Katoh

Publication Style

Journal name Biochemical Engineering Journal

Year　　

Volume, issue, pages

44(2-3) 289-291

Abstract

A single-chain variable fragment (scFv) antibody against bisphenol A was refolded using an antigen (bisphenol A)-coupled column. The refolding efficiency was compared with that used in dialysis. The refolding efficiency of the antigen-coupled column was about 50–60%, which was much higher than with dialysis, due to a decrease in the concentration of the refolding molecules and to the suppression of the aggregate formation.

DOI https://doi.org/10.1016/j.bej.2008.12.014